Once you have organized your lab and practiced micropipetting, it is time to run the DNA tests! Here is a quick guide for running on-farm Avian DNA tests.
Step 1: Prepare the reagents
Check this video tutorial for completing Step 1:
Reminder on how to organize the reagents area:
Cheat sheet for preparing the reagents:
All set for the next step!
Step 2: DNA extraction and PCR setup
Check this video tutorial for completing Step 2:
Reminder on how to organize the sampling area:
Cheat sheet for preparing the samples and setup PCR:
Step 3: Run PCR
Check this video for programing the miniPCR:
Reminder on how to organize the PCR area:
Cheat sheet on how to run PCR:
Step 4: Run the gel and visualize the results
Check these videos about how to prepare the gel, load the gel, and operate blueGel:
Cheat sheet on how to run the gel:
Step 5: Interpret the result
Check the video below and this blog with guidance regarding how to interpret the result.