Once you have organized your lab and practiced micropipetting, it is time to run the DNA tests! This blog is a quick guide for running on-farm Avian DNA tests.
Step 1: Prepare the reagents
Check this video tutorial for preparing the reagents:
Reminder on how to organize the reagents area:
How to prepare the reagents:
All set for the next step!
Step 2: DNA extraction and PCR setup
Check this video tutorial for completing the DNA extraction step:
Reminder on how to organize the sampling area:
How to extract DNA:
Step 3: Run PCR
Check this video for programing the miniPCR:
Check this video for setting up the PCR reaction:
Reminder on how to set up the PCR reaction:
Reminder on how to organize the PCR area:
Step 4: Run the gel and visualize the results
Check these videos about how to prepare the gel, load the gel, and operate blueGel:
Reminder on how to run the gel:
Step 5: Interpret the result
Check the video below and this blog with guidance regarding how to interpret the result.